thr77 for Dummies
thr77 for Dummies
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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has actually been noticed in many tumors, which results in chromosome instability, and so may possibly lead to tumorigenesis. This protein was discovered to affiliate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a important position in advertising mobile-cycle progression within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, have been explained. Two extra splice variants were being documented but comprehensive nucleotide sequence information and facts is just not however out there.
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Blocking peptides are peptides that bind specifically on the concentrate on antibody and block antibody binding. These peptide typically has the epitope recognized with the antibody.
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This tutorial outlines even more achievable optimization of cellular and immunoassay parameters to ensure the very best benefits are...
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This manual outlines additional achievable optimization of cellular and immunoassay parameters to be sure the absolute best benefits are...
Antibodies certain to the blocking peptide no more bind on the epitope about the focus on protein. By comparing the staining from your blocked antibody compared to the antibody alone, one can see which staining is specific.
Concentrate on Information and facts Cyclin E belongs to your remarkably conserved cyclin relatives, whose customers are characterized by a dramatic periodicity in protein abundance through the mobile cycle. Cyclins operate as regulators of CDK kinases. Distinct cyclins exhibit distinctive expression and degradation designs which contribute to the temporal coordination read more of each and every mitotic celebration. Cyclin E types a complex with and capabilities like a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates with the G1-S phase boundary and is particularly degraded as cells development via S section.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by the two antibodies...
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